plant expression vector pb 35s megfp Search Results


apoplast marker  (Addgene inc)
93
Addgene inc apoplast marker
Localization of mCherry fusion proteins transiently expressed in leaves of Nicotiana benthamiana . (A) OmAGAL2:mCherry, (E) ΔSP-OmAGAL2:mCherry, (I) SP:mCherry, and (M) mCherey were co-expressed with the <t>apoplast</t> marker At5g11420:pH-tdGFP (B, F, J, N). (C, D, G, H, K, L, O, P) Merged images of fluorescence from mCherry and GFP. Scale bars: 50 µm (A–C, E–G, I–K, M–O) and 20 µm (D, H, L, P).
Apoplast Marker, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plant+expression+vector+pb+35s+megfp/bio_rxiv__2021__06__16__448768-105-23-29?v=Addgene+inc
Average 93 stars, based on 1 article reviews
apoplast marker - by Bioz Stars, 2026-08
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vector ptc321  (Addgene inc)
96
Addgene inc vector ptc321
Localization of mCherry fusion proteins transiently expressed in leaves of Nicotiana benthamiana . (A) OmAGAL2:mCherry, (E) ΔSP-OmAGAL2:mCherry, (I) SP:mCherry, and (M) mCherey were co-expressed with the <t>apoplast</t> marker At5g11420:pH-tdGFP (B, F, J, N). (C, D, G, H, K, L, O, P) Merged images of fluorescence from mCherry and GFP. Scale bars: 50 µm (A–C, E–G, I–K, M–O) and 20 µm (D, H, L, P).
Vector Ptc321, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plant+expression+vector+pb+35s+megfp/pm30272678-315-1-42?v=Addgene+inc
Average 96 stars, based on 1 article reviews
vector ptc321 - by Bioz Stars, 2026-08
96/100 stars
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picslus0003od  (New England Biolabs)
99
New England Biolabs picslus0003od
Localization of mCherry fusion proteins transiently expressed in leaves of Nicotiana benthamiana . (A) OmAGAL2:mCherry, (E) ΔSP-OmAGAL2:mCherry, (I) SP:mCherry, and (M) mCherey were co-expressed with the <t>apoplast</t> marker At5g11420:pH-tdGFP (B, F, J, N). (C, D, G, H, K, L, O, P) Merged images of fluorescence from mCherry and GFP. Scale bars: 50 µm (A–C, E–G, I–K, M–O) and 20 µm (D, H, L, P).
Picslus0003od, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plant+expression+vector+pb+35s+megfp/bio_rxiv__2020__09__27__315812-42-9-17?v=New+England+Biolabs
Average 99 stars, based on 1 article reviews
picslus0003od - by Bioz Stars, 2026-08
99/100 stars
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puc19 35s cg2lysm flag  (New England Biolabs)
97
New England Biolabs puc19 35s cg2lysm flag
Localization of mCherry fusion proteins transiently expressed in leaves of Nicotiana benthamiana . (A) OmAGAL2:mCherry, (E) ΔSP-OmAGAL2:mCherry, (I) SP:mCherry, and (M) mCherey were co-expressed with the <t>apoplast</t> marker At5g11420:pH-tdGFP (B, F, J, N). (C, D, G, H, K, L, O, P) Merged images of fluorescence from mCherry and GFP. Scale bars: 50 µm (A–C, E–G, I–K, M–O) and 20 µm (D, H, L, P).
Puc19 35s Cg2lysm Flag, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plant+expression+vector+pb+35s+megfp/pmc09982014-90-26-68?v=New+England+Biolabs
Average 97 stars, based on 1 article reviews
puc19 35s cg2lysm flag - by Bioz Stars, 2026-08
97/100 stars
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reticulocyte lysate  (Promega)
90
Promega reticulocyte lysate
Localization of mCherry fusion proteins transiently expressed in leaves of Nicotiana benthamiana . (A) OmAGAL2:mCherry, (E) ΔSP-OmAGAL2:mCherry, (I) SP:mCherry, and (M) mCherey were co-expressed with the <t>apoplast</t> marker At5g11420:pH-tdGFP (B, F, J, N). (C, D, G, H, K, L, O, P) Merged images of fluorescence from mCherry and GFP. Scale bars: 50 µm (A–C, E–G, I–K, M–O) and 20 µm (D, H, L, P).
Reticulocyte Lysate, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plant+expression+vector+pb+35s+megfp/pmc00548017-43-13-15?v=Promega
Average 90 stars, based on 1 article reviews
reticulocyte lysate - by Bioz Stars, 2026-08
90/100 stars
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35s pbs yfp attr  (Thermo Fisher)
99
Thermo Fisher 35s pbs yfp attr
Localization of mCherry fusion proteins transiently expressed in leaves of Nicotiana benthamiana . (A) OmAGAL2:mCherry, (E) ΔSP-OmAGAL2:mCherry, (I) SP:mCherry, and (M) mCherey were co-expressed with the <t>apoplast</t> marker At5g11420:pH-tdGFP (B, F, J, N). (C, D, G, H, K, L, O, P) Merged images of fluorescence from mCherry and GFP. Scale bars: 50 µm (A–C, E–G, I–K, M–O) and 20 µm (D, H, L, P).
35s Pbs Yfp Attr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plant+expression+vector+pb+35s+megfp/pm25292417-228-14-29?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
35s pbs yfp attr - by Bioz Stars, 2026-08
99/100 stars
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plant expression vector pb 35s megfp  (Addgene inc)
93
Addgene inc plant expression vector pb 35s megfp
Localization of mCherry fusion proteins transiently expressed in leaves of Nicotiana benthamiana . (A) OmAGAL2:mCherry, (E) ΔSP-OmAGAL2:mCherry, (I) SP:mCherry, and (M) mCherey were co-expressed with the <t>apoplast</t> marker At5g11420:pH-tdGFP (B, F, J, N). (C, D, G, H, K, L, O, P) Merged images of fluorescence from mCherry and GFP. Scale bars: 50 µm (A–C, E–G, I–K, M–O) and 20 µm (D, H, L, P).
Plant Expression Vector Pb 35s Megfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plant+expression+vector+pb+35s+megfp/pmc09618770-278-8-12?v=Addgene+inc
Average 93 stars, based on 1 article reviews
plant expression vector pb 35s megfp - by Bioz Stars, 2026-08
93/100 stars
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35s]met  (HARTMANN ANALYTIC)
90
HARTMANN ANALYTIC 35s]met
Localization of mCherry fusion proteins transiently expressed in leaves of Nicotiana benthamiana . (A) OmAGAL2:mCherry, (E) ΔSP-OmAGAL2:mCherry, (I) SP:mCherry, and (M) mCherey were co-expressed with the <t>apoplast</t> marker At5g11420:pH-tdGFP (B, F, J, N). (C, D, G, H, K, L, O, P) Merged images of fluorescence from mCherry and GFP. Scale bars: 50 µm (A–C, E–G, I–K, M–O) and 20 µm (D, H, L, P).
35s]Met, supplied by HARTMANN ANALYTIC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plant+expression+vector+pb+35s+megfp/10__1074_slash_jbc__m109__032631-88-26-27?v=HARTMANN+ANALYTIC
Average 90 stars, based on 1 article reviews
35s]met - by Bioz Stars, 2026-08
90/100 stars
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binary vector psb161  (Addgene inc)
93
Addgene inc binary vector psb161
Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the <t>pSB161</t> plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.
Binary Vector Psb161, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plant+expression+vector+pb+35s+megfp/bio_rxiv__2025__09__10__675371-157-1-29?v=Addgene+inc
Average 93 stars, based on 1 article reviews
binary vector psb161 - by Bioz Stars, 2026-08
93/100 stars
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pfretgc 35s  (Proteintech)
96
Proteintech pfretgc 35s
Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the <t>pSB161</t> plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.
Pfretgc 35s, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plant+expression+vector+pb+35s+megfp/bio_rxiv__2024__01__09__574788-92-20-51?v=Proteintech
Average 96 stars, based on 1 article reviews
pfretgc 35s - by Bioz Stars, 2026-08
96/100 stars
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gmfer1hap2 gmfer1 flag 35s gfp hap2 recombinant vector  (Vector Laboratories)
96
Vector Laboratories gmfer1hap2 gmfer1 flag 35s gfp hap2 recombinant vector
Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the <t>pSB161</t> plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.
Gmfer1hap2 Gmfer1 Flag 35s Gfp Hap2 Recombinant Vector, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plant+expression+vector+pb+35s+megfp/pm40365869-355-7-10?v=Vector+Laboratories
Average 96 stars, based on 1 article reviews
gmfer1hap2 gmfer1 flag 35s gfp hap2 recombinant vector - by Bioz Stars, 2026-08
96/100 stars
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pcambia-3300-35s-nterm-y155n-u  (Nagai Nori USA INC)
90
Nagai Nori USA INC pcambia-3300-35s-nterm-y155n-u
Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the <t>pSB161</t> plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.
Pcambia 3300 35s Nterm Y155n U, supplied by Nagai Nori USA INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plant+expression+vector+pb+35s+megfp/pmc04311218-436-1-18?v=Nagai+Nori+USA+INC
Average 90 stars, based on 1 article reviews
pcambia-3300-35s-nterm-y155n-u - by Bioz Stars, 2026-08
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Image Search Results


Localization of mCherry fusion proteins transiently expressed in leaves of Nicotiana benthamiana . (A) OmAGAL2:mCherry, (E) ΔSP-OmAGAL2:mCherry, (I) SP:mCherry, and (M) mCherey were co-expressed with the apoplast marker At5g11420:pH-tdGFP (B, F, J, N). (C, D, G, H, K, L, O, P) Merged images of fluorescence from mCherry and GFP. Scale bars: 50 µm (A–C, E–G, I–K, M–O) and 20 µm (D, H, L, P).

Journal: bioRxiv

Article Title: Localization of planteose hydrolysis during seed germination of Orobanche minor

doi: 10.1101/2021.06.16.448768

Figure Lengend Snippet: Localization of mCherry fusion proteins transiently expressed in leaves of Nicotiana benthamiana . (A) OmAGAL2:mCherry, (E) ΔSP-OmAGAL2:mCherry, (I) SP:mCherry, and (M) mCherey were co-expressed with the apoplast marker At5g11420:pH-tdGFP (B, F, J, N). (C, D, G, H, K, L, O, P) Merged images of fluorescence from mCherry and GFP. Scale bars: 50 µm (A–C, E–G, I–K, M–O) and 20 µm (D, H, L, P).

Article Snippet: Transient expression was conducted by co-inoculation of Agrobacterium tumefaciens strain GV3101 cultures carrying each construct with those carrying the vector pMDC-At5g11420:pH-tdGFP as an apoplast marker , and pDGB3alph2_35S:P19:Tnos (GB1203, Addgene #68214) in leaves of Nicotiana benthamiana . mCherry and green fluorescent protein (GFP) were excited at 555 and 488 nm, and observed in the range of 570–600 and 490–520 nm, respectively, using a LSM700 laser scanning confocal microscope (Carl Zeiss, Jena, Germany).

Techniques: Marker, Fluorescence

Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the pSB161 plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.

Journal: bioRxiv

Article Title: Systematic Optimization Enables Near-Perfect In Vitro Transformation Efficiencies for Spirodela polyrhiza (Greater Duckweed)

doi: 10.1101/2025.09.10.675371

Figure Lengend Snippet: Transient transformation of S. polyrhiza using Agrobacterium tumefaciens. (A) Transformed fronds were cultured in liquid medium following co-cultivation. Only fronds treated with A. tumefaciens strain GV3101 exhibited signs of successful transient transformation, whereas those treated with LBA4404 showed no detectable response. (B) Histochemical GUS staining of S. polyrhiza callus tissue transformed with GV3101 harboring the pSB161 plasmid. Multiple, blue-stained foci (indicated by red arrows) represent regions of GUS reporter gene expression, confirming successful transient transformation and efficiency is 95%.

Article Snippet: The binary vector pSB161 (pL2_pSB90_2×35S::GUS::tMAS), which harbors a GUS reporter gene with introns under the control of a double 35S promoter, was obtained from Erin Cram and Carolyn Lee-Parsons (Addgene plasmid #123197; http://n2t.net/addgene:123197 ; RRID:Addgene_123197).

Techniques: Transformation Assay, Cell Culture, Staining, Plasmid Preparation, Gene Expression